Transcriptomics and Proteomics in Zebrafish

This workshop is organized in the framework of the European ZF-MODLES project: ”Zebrafish Models for Human Development and Disease”. The workshop will give an introduction to transcriptome microarray analysis and proteomics and is primarily intended for graduate students, postdocs and advanced technicians using zebrafish as research model. The workshop is aimed at providing knowledge on the use of different microarray platforms, RNA labelling techniques, protein extraction methods and 2D-electrophoresis, mass spectrometry, experimental design of genomic studies, statistical analysis of genomic data, linkage of datasets to the zebrafish genome and the use of public databases for storage of genomic data. The practical part will include RNA labeling, quality control and hybridization of oligonucleotide microarrays. Furthermore, the participants will be able to acquire practical experience with bioinformatic methods applied in genomics and proteomics.

Instructors:

Microarray expression profiling

Georg Otto, Max-Planck Institute of Developmental Biology, Germany

Annemarie Meijer and Herman Spaink, Institute of Biology, Leiden University, the Netherlands

Bioinformatics

Fons Verbeek, Leiden Institute of Advanced Computer Science, Leiden University, the Netherlands

Mario Caccamo, The Wellcome Trust Sanger Institute, Cambridge, United Kingdom

Marc Saric, Max-Planck Institute of Developmental Biology, Germany

Proteomics

Carl-Philipp Heisenberg, Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

Astrid Bruckmann and Enrique Salas, Institute of Biology, Leiden University, the Netherlands